With the worldwide strive of the automotive industry to evaluate and develop alternative mobility concepts, the incorporation of alternative fuels into the current fuel pool gains a strong position in the race to the future. The understanding of the interaction of these novel fuels with engine components, especially sealing materials, is mandatory for the long-life operation of an engine system. Current analytical techniques to determine the impact of Biofuels on sealing material properties lack strong correlation with engine performance in the field. In this paper, major drawbacks of current lab techniques used for evaluation of different Biofuels and OillBiofuel mixtures are highlighted. For example, current lab test results with oil contaminated with 10vol% E85 yielded 145% increase in volume for a standard Polyacrylate Rubber (ACM) under moderate conditions, clearly signaling a destruction of the sealing function, whereas, Original Equipment Manufacturers (OEM) do not report significant issues during engine tests with E85. Therefore, a new generation of modular lab scale test equipment has been developed, that enables the evaluation sealing material compatibility with a wide range of ethanol- and Biodiesel based Biofuels and OillBiofuel mixtures under application-like conditions. The results show that under a broad range of conditions, the maximum volume swell of an ACM sealing material in an engine environment does not exceed 14%, which is more in line with field experience.